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Cancer Research

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JoVE Journal Cancer Research
Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs
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Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

Article DOI: 10.3791/60687-v • 10:32 min • December 19th, 2019
December 19th, 2019 •
1, 2, 1
1Department of Molecular and Cellular Physiology, Albany Medical College, 2Department of Neuroscience and Experimental Therapeutics, Albany Medical College

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The described approach combines experimental tail vein metastasis assays with in vivo live animal imaging to allow real-time monitoring of breast cancer metastasis formation and growth in addition to the quantification of metastasis number and size in the lungs.

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Tail Vein Metastasis Assays Real-time In Vivo Imaging Breast Cancer Metastatic Colonization Burden In The Lungs Candidate Gene Role In Metastatic Colonization And Growth Real-time Monitoring Of Metastasis Quantification Of Metastasis Number And Size Luciferase Viral Supernatant Fluorescent Protein Viral Supernatant Hexadimethrine Bromide Trypsinize Cells Selection Media ZsGreen Fluorescent Protein Expression
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