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Anvendelse af fryseoptøede embryoner til højeffektiv produktion af genetisk modificerede mus
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Anvendelse af fryseoptøede embryoner til højeffektiv produktion af genetisk modificerede mus

Article DOI: 10.3791/60808-v • 06:46 min • April 2nd, 2020
April 2nd, 2020 •
*1,2,3, *4,5, 6,7, 8,9,10, 8,11, 3, 8,9,10,12,13, 1
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo
* These authors contributed equally

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Her præsenterer vi en modificeret metode til kryopræservering af encellede embryoner samt en protokol, der kobler brugen af fryseoptøede embryoner og elektroporation til effektiv generation af genetisk modificerede mus.

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Genetik CRISPR/Cas9 encellede embryo kryopræservering elektroporation genetisk modificerede mus GM genomredigering FBS
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