Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Indel Detection following CRISPR/Cas9 Mutagenesis …
JoVE Journal
Genetics

Un abonnement à JoVE est nécessaire pour afficher ce contenu.

JoVE Journal Genetics
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
Journal (Genetics)
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
Journal (Genetics)
Lentiviral Mediated Gene Silencing in Human Pseudoislet Prepared in Low Attachment Plates
 

Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti

Article DOI: 10.3791/63008-v • 05:30 min • September 10th, 2021
September 10th, 2021 •
1, 1, 1, 1, 1
1Department of Entomology and Agrilife Research, Texas A&M University

Chapitres

résumé
Automatic Translation

This article details a protocol for rapid identification of indels induced by CRISPR/Cas9 and selection of mutant lines in the mosquito Aedes aegypti using high-resolution melt analysis.

Tags

Indel Detection CRISPR/Cas9 Mutagenesis High-resolution Melt Analysis Aedes Aegypti Mutants Protocol Technique Organisms Primer Design Verification HRMA Gradient PCR Master Mix Non-template Control (NTC) Template Sample Mix Cycling Parameters Thermal Melt Profiles DNA Release Reagent Dilution Buffer Genotyping Multichannel Pipet G1 Mosquitoes Wild-type Mosquitoes Hind Legs
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter