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Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit

Article DOI: 10.3791/636-v • 12:39 min • January 22nd, 2008
January 22nd, 2008 •
1, 1
1Department of Developmental and Cell Biology, University of California, Irvine (UCI)

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The complete genotyping of a mouse tail sample, including tissue digestion and PCR readout, is done in one and a half hours using Sigma's SYBR Green Extract-N-Amp Tissue PCR kit.

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Rapid Genotyping Mouse Tissue Sigma's Extract-N-Amp Tissue PCR Kit DNA Detection PCR Amplification Electrophoretic Gel Tissue Sample Genotype PCR-ready DNA Tissue Preparation Genotyping Process Mouse Tail Sample SYBR Green Extract-N-Amp Tissue PCR Kit DNA Extraction Real-time PCR Amplification Positive Sample Identification Prompt Genotype Identification
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