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6 articles published in JoVE
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A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
Mark J. Bailey1,2, Felix Broecker1, Paul E. Leon1,2, Gene S. Tan3
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease, J. Craig Venter Institute
We describe a method to measure the activation of Fc-mediated effector functions by antibodies that target the influenza virus hemagglutinin. This assay can also be adapted to assess the ability of monoclonal antibodies or polyclonal sera targeting other viral surface glycoproteins to induce Fc-mediated immunity.
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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
Evan Bardot1,2,3, Nikos Tzavaras4, Deanna L. Benson5,6, Nicole C. Dubois1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai
Here, we describe a protocol for whole mount immunofluorescence and image-based quantitative volumetric analysis of early stage mouse embryos. We present this technique as a powerful approach to qualitatively and quantitatively assess cardiac structures during development, and propose that it may be widely adaptable to other organ systems.
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Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
Lisa Mohr1, Marc Carceles-Cordon1, Jungreem Woo1, Carlos Cordon-Cardo1, Josep Domingo-Domenech1, Veronica Rodriguez-Bravo1,2
1Department of Pathology, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, 2Department of Oncological Sciences, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai
Resistance to cancer therapies contributes to disease progression and death. Determining the mechanistic underpinnings of resistance is crucial for improving therapeutic response. This manuscript details the protocol to generate taxane-resistant cell models of prostate cancer (PC) to help dissecting the pathways involved in progression to Docetaxel resistance in PC patients.
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Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Paul E. Leon1,2, Teddy John Wohlbold1,2, Wenqian He1,2, Mark J. Bailey1,2, Carole J. Henry3, Patrick C. Wilson3, Florian Krammer1, Gene S. Tan1
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago
We describe a method by which we identify critical residues required for the binding of human or murine monoclonal antibodies that target the viral hemagglutinin of influenza A viruses. The protocol can be adapted to other virus surface glycoproteins and their corresponding neutralizing antibodies.
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A Comprehensive Procedure to Evaluate the In Vivo Performance of Cancer Nanomedicines
Jun Tang1, Carlos Pérez-Medina1,2, Yiming Zhao2, Ahmad Sadique1, Willem J. M. Mulder2, Thomas Reiner1
1Department of Radiology, Memorial Sloan Kettering Cancer Center, 2Translational and Molecular Imaging Institute, Icahn School of Medicine at Mount Sinai
The poor understanding of the in vivo performance of nanomedicines stymies their clinical translation. Procedures to evaluate the in vivo behavior of cancer nanomedicines at systemic, tissue, single-cell, and subcellular levels in tumor-bearing immunocompetent mice are described here. This approach may help researchers to identify promising cancer nanomedicines for clinical translation.
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