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Cancer Research

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JoVE Journal Cancer Research
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Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Article DOI: 10.3791/57011-v • 07:18 min • June 13th, 2019
June 13th, 2019 •
1, 1, 1, 1, 1
1Department of Pathology, Icahn School of Medicine at Mount Sinai

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Summary
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We describe a detailed protocol for the isolation of tumor-initiating cells from human sarcoma patient-derived xenografts by fluorescence-activated cell sorting, using human leukocyte antigen-1 (HLA-1) as a negative marker, and for the further validation and characterization of these HLA-1-negative tumor-initiating cells.

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Isolation Characterization Tumor-initiating Cells Sarcoma Patient-derived Xenografts HLA-I Epigenetically Downregulated Cancer Cells Stem Cell Properties Tumor-initiating Capacity Immune Escape Survival Advantages HLA-I Negativity Functional Marker Sudeh Izadmehr Postdoc Laboratory Sample Acquisition Tissue Petri Dish Sterilized Bio Safety Cabinet PBS Scalpel Triturate Fragments Cell Strainer Conical Tube Mince Dissociated Centrifugation Pellet Resuspension Hemolysis Buffer
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