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DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation

Article DOI: 10.3791/1520-v • 15:56 min • October 19th, 2009
October 19th, 2009 •
1, 1, 1, 1
1Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles

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We describe detailed procedures for the efficient transfection of plasmid DNA into the fibers of foot muscles of live mice using electroporation and the subsequent visualization of protein expression using fluorescence microscopy.

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DNA Transfection Mammalian Skeletal Muscles In Vivo Electroporation Cell Biology Transgenic Proteins Fluorescently Tagged Constructs Mutant Variants In Vivo Transfection Plasmids Specific Tissues Liver Skeletal Muscle Brain Flexor Digitorum Brevis Interosseus Muscles Adult Mice Experimental Parameters Soluble Proteins Structural Proteins Membrane Proteins EGFP ECFP3 Calpain FKBP12 β2a-DHPR Minidystrophin α-actinin α1s-DHPR RyR1 Cardiac Na/Ca2+ Exchanger NaV1.4 Na Channel SERCA1
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