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Journal / Biology / Modified Mouse Embryonic Stem Cell based Assay…
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Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency
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Modified Mouse Embryonic Stem Cell based Assay for Quantifying Cardiogenic Induction Efficiency

Article DOI: 10.3791/2656-v • 08:08 min • April 22nd, 2011
April 22nd, 2011 •
1, 1, 1, 1,2,3,4
1Division of Cardiovascular Medicine, Department of Medicine, Vanderbilt University School of Medicine, 2Department of Pharmacology, Vanderbilt University School of Medicine, 3Vanderbilt Institute of Chemical Biology, Vanderbilt University School of Medicine, 4Research Medicine, Veterans Administration TVHS

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We describe the use of a mouse ES cell based assay to identify critical time windows for Wnt/β-catenin and BMP signal activation during cardiogenic induction. The method provides a standardized platform that reliably quantifies cardiogenic efficiency, and it is applicable to the study of other cell lineages.

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Modified Mouse Embryonic Stem Cell Assay Quantifying Cardiogenic Induction Efficiency Pluripotent Stem Cells Key Signaling Pathways Temporal Regulation Spatial Regulation Differentiation Efficiency Wnt/β-catenin Signaling BMP Signaling Contracting Embryonic Bodies 96-well Plate Format Cardiogenic Efficiency Modulator Treatments Time Course Cell Lineages High Throughput Assay Automated Assay
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