Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / High Throughput Screening of Fungal Endoglucanase …
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Journal (Biology)
Profiling of Methyltransferases and Other S-adenosyl-L-homocysteine-binding Proteins by Capture Compound Mass Spectrometry (CCMS)
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
Journal (Biology)
Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker
 
Click here for the English version

High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Article DOI: 10.3791/2942-v • 06:16 min • August 13th, 2011
August 13th, 2011 •
1, 2
1Department Of Chemistry and Chemical Engineering, California Institute of Technology, 2Chemistry and Chemical Engineering, California Institute of Technology

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

We describe a low cost, high throughput method to screen for fungal endoglucanase activity in E. coli. The method relies on a simple visual readout of substrate degradation, does not require enzyme purification, and is highly scalable. This allows for the rapid screening of large libraries of enzyme variants.

Tags

High Throughput Screening Fungal Endoglucanase Activity Escherichia Coli Cellulase Enzymes Cellulose Hydrolysis Fuel Alcohols Enzymatic Hydrolysis Cellulosic Biomass Renewable Energy Engineer Cellulases Fungal Cellulases Mutant Cellulases Library Genetic Tools For Native Organisms Expressing Enzymes In Heterologous Hosts E. Coli Expression Industrial Fungus H. Jecorina Cellulases Secretion Macrophomina Phaseolina Phanerochaete Chrysosporium High Throughput Screening Congo Red Dye
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter