Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Monitoring Plasmid Replication in Live Mammalian…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Biology
Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
Journal (Biology)
Metabolic Labeling and Membrane Fractionation for Comparative Proteomic Analysis of Arabidopsis thaliana Suspension Cell Cultures
Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE
Journal (Biology)
Generation of and Comparison Between Patient-Derived Gastric Organoids from Different Regions of the Stomach (Video) | JoVE
 

Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

Article DOI: 10.3791/4305-v • 12:34 min • December 13th, 2012
December 13th, 2012 •
1, 1, 1
1Department of Oncology, University of Wisconsin - Madison

Capitoli

Riepilogo
Automatic Translation

A method of observing individual DNA molecules in live cells is described. The technique is based on the binding of a fluorescently tagged lac repressor protein to binding sites engineered into the DNA of interest. This method can be adapted to follow many recombinant DNAs in live cells over time.

Tags

Plasmid Replication Live Mammalian Cells Fluorescence Microscopy Gamma-herpesviruses Epstein-Barr Virus Kaposi's Sarcoma-associated Herpesvirus Licensed Replication Viral Protein Cellular Replication Machinery Centromeres Southern Blotting Fluorescence In Situ Hybridization (FISH) Quantitative PCR Visualizing Plasmids Lactose Repressor Protein LacO Sequence
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter