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Journal / Biology / 人类多能干细胞的高效分化为心肌细胞，并鉴定流式细胞仪…
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人类多能干细胞的高效分化为心肌细胞，并鉴定流式细胞仪
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人类多能干细胞的高效分化为心肌细胞，并鉴定流式细胞仪

Article DOI: 10.3791/52010-v • 13:13 min • September 23rd, 2014
September 23rd, 2014 •
*1, *2, 1, 1, 3, 2, 4,5, 1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
* These authors contributed equally

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该文章描述的详细方法通过选择性调节Wnt通路，随后通过流式细胞仪的参考标记分析来评估群体的均匀性和同一性的有效分化人多能干细胞分化为心肌细胞。

Tags

细胞生物学，第91，人类诱导多能干细胞，流式细胞仪，定向分化，心肌细胞，IRX4，TNNI3，TNNT2，MCL2v，MLC2a
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