Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Generation of Integration-free Induced…
JoVE Journal
Developmental Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto.

JoVE Journal Developmental Biology
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
Journal (Developmental Biology)
Retrograde Tracing of Drosophila Embryonic Motor Neurons Using Lipophilic Fluorescent Dyes
A Drosophila Model to Study Wound-induced Polyploidization
Journal (Developmental Biology)
A Drosophila Model to Study Wound-induced Polyploidization
 

Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

Article DOI: 10.3791/55091-v • 09:45 min • January 1st, 2017
January 1st, 2017 •
1, 1, 2, 2, 1,2, 2, 3, 1, 1, 1,4,5,6,7, 1,2
1State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 2Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 3Department of Orthopaedic Surgery, Loma Linda University, 4Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, 5Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, 6Collaborative Innovation Center for Cancer Medicine, 7Tianjin Key Laboratory of Blood Cell Therapy and Technology

Capitoli

Riepilogo
Automatic Translation

This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.

Tags

Induced Pluripotent Stem Cells Peripheral Blood Mononuclear Cells Episomal Vectors Reprogramming Protocol Stem Cell Field Disease Monitoring Drug Screening Radio Medicine Plasma Quality Contaminants Endofree Kits Passing Gene Culture Media PB Sample PBS Buffer Ficoll Layer Centrifugation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter