Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Rupak Pathak; Igor Koturbash; Martin Hauer-Jensen

doi:10.3791/55162

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Rilevazione di Inter-aberrazioni cromosomiche stabili per fluorescenza multipla In Situ Ibridazione (mFISH) e spettrale cariotipo (SKY) in topi irradiati

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence <em>In Situ</em> Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

JoVE Journal
Genetica

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JoVE Journal Genetica

Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Rilevazione di Inter-aberrazioni cromosomiche stabili per fluorescenza multipla In Situ Ibridazione (mFISH) e spettrale cariotipo (SKY) in topi irradiati

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9,926 Views

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10:14 min

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January 11, 2017

DOI:

10.3791/55162-v

DOI:

10.3791/55162-v

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10:14 min

January 11, 2017

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9880 Views

Rupak Pathak¹, Igor Koturbash², Martin Hauer-Jensen^1,3

¹Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy,University of Arkansas for Medical Sciences ²Department of Environmental Health, Fay W. Boozman School of Public Health,University of Arkansas for Medical Sciences ³Surgical Service,Central Arkansas Veterans Healthcare System

Summary

The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.