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Journal / Developmental Biology / Adult Mouse Digit Amputation and Regeneration: A…
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Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification

Article DOI: 10.3791/59749-v • 09:17 min • July 12th, 2019
July 12th, 2019 •
1, 1, 1, 1, 2, 3, 1, 1, 1, 1, 1, 1
1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 2Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 3Department of Neurosurgery, University of Mississippi Medical Center

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Here, we present a protocol of adult mouse terminal phalanx amputation to investigate mammalian blastema formation and intramembranous ossification, analyzed by fluorescent immunohistochemistry and sequential in-vivo microcomputed tomography.

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Mouse Digit Amputation Mammalian Blastema Formation Intramembranous Ossification P3 Amputation Regenerative Medicine Cell Populations Revascularization Bone Stabilization Device CD-1 Mouse Surgical Procedure Bupivacaine Anesthesia
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