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Fremstilling af virusberiget inokulum til oral infektion af honningbier (Apis mellifera)
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Fremstilling af virusberiget inokulum til oral infektion af honningbier (Apis mellifera)

Article DOI: 10.3791/61725-v • 06:41 min • August 26th, 2020
August 26th, 2020 •
1, 2, 1
1Department of Entomology, University of Illinois, 2Department of Microbiology, Center for Scientific Research and Higher Education of Ensenada (CICESE)

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Her beskriver vi to protokoller: for det første at formere, udtrække, rense og kvantificere store mængder honningbi-ikke-indhyllede viruspartikler, herunder en metode til fjernelse af honningbipupper og for det andet at teste virkningerne af virusinfektion ved hjælp af et meget repeterbart burbioassay med høj gennemstrømning.

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Biologi udgave 162 honningbi Apis mellifera bivirus oral infektion burbioassay ekstraktion af virale partikler
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