Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Single Cell Electroporation in vivo within the…
JoVE Journal
Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Biology
Single Cell Electroporation in vivo within the Intact Developing Brain
Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat
Journal (Biology)
Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat
Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
Journal (Biology)
Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples
 
Click here for the English version

Single Cell Electroporation in vivo within the Intact Developing Brain

Article DOI: 10.3791/705-v • 13:31 min • July 11th, 2008
July 11th, 2008 •
1,2, 1,2
1Brain Research Centre, University of British Columbia - UBC, 2Department of Cellular and Physiological Sciences, University of British Columbia - UBC

Capitoli

Riepilogo
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Single-cell electroporation (SCE) is a specialized technique allowing delivery of DNA or other macromolecules into individual cells within intact tissue, including in vivo preparations. Here we detail the procedure for SCE of a fluorescent dye or plasmid DNA into neurons within the intact brain of the Xenopus laevis tadpole.

Tags

Single-cell Electroporation In Vivo Intact Developing Brain DNA Delivery Macromolecules Individual Cells Intact Tissue Experimental Manipulations Cell-autonomous Effects Global Treatments In Vivo Imaging Techniques Fluorescent Markers Cellular Morphology Cell Growth Intracellular Events Xenopus Laevis Tadpoles Video Article Procedure For SCE Fluorescent Dye Plasmid DNA Neurons Albino Xenopus Tadpole Yield Optimization Two-photon Fluorescence Imaging
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter