Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

Carine Fillebeen; Nicole Wilkinson; Kostas Pantopoulos

doi:10.3791/52230

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生物学

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Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

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生物学

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JoVE Journal 生物学

Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

53,085 Views

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12:44 min

•

December 03, 2014

DOI:

10.3791/52230-v

DOI:

10.3791/52230-v

•

12:44 min

December 03, 2014

•

44 Views

Carine Fillebeen^1,2, Nicole Wilkinson^1,2, Kostas Pantopoulos^1,2

¹Lady Davis Institute for Medical Research,Jewish General Hospital, ²医学部,McGill University

概要

Here we present a protocol to analyze RNA/protein interactions. The electrophoretic mobility shift assay (EMSA) is based on the differential migration of RNA/protein complexes and free RNA during native gel electrophoresis. By using a radiolabeled RNA probe, RNA/protein complexes can be visualized by autoradiography.

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Electrophoretic Mobility Shift Assay (EMSA) for the Study of RNA-Protein Interactions: The IRE/IRP Example

概要

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