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Chelating Agents: Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of Coordination complexes with Metals.

Sample Preparation for Analytical Preparation

JoVE 10205

Source: Laboratory of Dr. B. Jill Venton - University of Virginia

Sample preparation is the way in which a sample is treated to prepare for analysis. Careful sample preparation is critical in analytical chemistry to accurately generate either a standard or unknown sample for a chemical measurement. Errors in analytical chemistry methods are categorized as random or systematic. Random errors are errors due to change and are often due to noise in instrument. Systematic errors are due to investigator or instrumental bias, which introduces an offset in the measured value. Errors in sample preparation are systematic errors, which will propagate through analysis, causing uncertainty or inaccuracies through improper calibration curves. Systematic errors can be eliminated through correct sample preparation and proper use of the instrument. Poor sample preparation can also sometimes cause harm to the instrument.


 Analytical Chemistry

One Minute, Sub-One-Watt Photothermal Tumor Ablation Using Porphysomes, Intrinsic Multifunctional Nanovesicles

1Department of Pharmaceutical Sciences, University of Toronto, 2The Institute of Biomaterials and Biomedical Engineering, University of Toronto, 3Ontario Cancer Institute, Campbell Family Institute For Cancer Research and Techna Institute, 4Department of Biomedical Engineering, University at Buffalo, The State University of New York

JoVE 50536


 Bioengineering

Coordination Chemistry Complexes

JoVE 10179

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry

Transition metals are found everywhere from vitamin supplements to electroplating baths. Transition metals also make up the pigments in many paints and compose all minerals. Typically, transition metals are found in the cationic form since they readily oxidize, or lose electrons, and are surrounded by electron donors called ligands. These ligands do not form ionic or covalent bonds with the metal center, rather they take on a third type of bond known as coordinate-covalent. The coordinate-covalent bond between a ligand and a metal is dynamic, meaning that ligands are continuously exchanging and re-coordinating around the metal center. The identities of both the metal and the ligand dictates which ligands will bond preferentially over another. In addition, color and magnetic properties are also due to the types of complexes that are formed. The coordination compounds that form are analyzed using a variety of instruments and tools. This experiment explores why so many complexes are possible and uses a spectrochemical (color and chemical) method to help identify the type of coordination complex that forms.


 General Chemistry

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

1Department of Chemical Engineering, McGill University, 2Michael Smith Laboratories & Department of Chemical and Biological Engineering, University of British Columbia, 3Michael Smith Laboratories & Department of Pharmaceutical Sciences, University of British Columbia

JoVE 55280


 Bioengineering

Methods for Analyzing the Impacts of Natural Uranium on in vitro Osteoclastogenesis

1UMR E-4320 TIRO-MATOs CEA/DRF/BIAM, Université Nice Sophia-Antipolis, 2UMR 7272 Institut de Chimie de Nice CNRS, Université Nice Sophia-Antipolis, 3CEA, Direction de la Recherche Fondamentale (DRF), Biosciences and Biotechnologies Institute (BIAM)

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JoVE 56499


 JoVE In-Press

Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs

1Sinclair Centre for Regenerative Medicine, Ottawa Hospital Research Institute, 2University of Ottawa, 3Department of Neonatology and Pediatric Critical Care Medicine, Medical Faculty and University Hospital Carl Gustav Carus, Technische Universität Dresden, 4DFG Research Center and Cluster of Excellence for Regenerative Therapies (CRTD), Technische Universität, Dresden, 5Children's Hospital of Eastern Ontario Research Institute

JoVE 53782


 Biology

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Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

1The Sheikh Zayed Institute for Pediatric Surgical Innovation, Children's National Medical Center, 2Fischell Department of Bioengineering, University of Maryland, 3Department of Radiology, George Washington University, 4Department of Pediatrics, George Washington University

JoVE 52621


 Bioengineering

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Whole Genome Sequencing of Candida Glabrata for Detection of Markers of Antifungal Drug Resistance

1Centre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, 2Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, 3Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, 4Department of Microbiology and Infectious Diseases, Canberra Hospital and Health Services, Australian National University Medical School, 5Infection Management Services, Princess Alexandra Hospital, 6Department of Microbiology and Infectious Diseases, St. Vincent's Hospital, 7Department of Infectious Diseases, Peter MacCallum Cancer Centre

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JoVE 56714


 JoVE In-Press

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Fabrication and Characterization of Griffithsin-modified Fiber Scaffolds for Prevention of Sexually Transmitted Infections

1Department of Chemistry, University of Louisville, 2Department of Pharmacology and Toxicology, University of Louisville, 3Center for Predictive Medicine, University of Louisville, 4Department of Microbiology and Immunology, University of Louisville, 5Department of Bioengineering, University of Louisville

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JoVE 56492


 JoVE In-Press

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