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Complex Mixtures: Mixtures of many components in inexact proportions, usually natural, such as Plant extracts; Venoms; and Manure. These are distinguished from Drug combinations which have only a few components in definite proportions.

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

1Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, 2Department of Soil, Water and Environmental Science, University of Arizona, 3Department of Earth Ocean and Atmospheric Sciences, Florida State University, 4Bruker Daltonics Inc., 5Biological Sciences Division, Pacific Northwest National Laboratory

JoVE 59035

 Environment

Two-Dimensional Gel Electrophoresis

JoVE 5686

Two-dimensional gel electrophoresis (2DGE) is a technique that can resolve thousands of biomolecules from a mixture. This technique involves two distinct separation methods that have been coupled together: isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This physically separates compounds across two axes of a gel by their isoelectric points…

 Biochemistry

Purification of a Total Lipid Extract with Column Chromatography

JoVE 10159

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst


The product of an organic solvent extraction, a total lipid extract (TLE), is often a complex mixture of hundreds, if not thousands, of different compounds. The researcher is often only interested in a handful of compounds. The compounds of interest may belong to …

 Earth Science

Sample Preparation for Analytical Characterization

JoVE 10205

Source: Laboratory of Dr. B. Jill Venton - University of Virginia


Sample preparation is the way in which a sample is treated to prepare for analysis. Careful sample preparation is critical in analytical chemistry to accurately generate either a standard or unknown sample for a chemical measurement. Errors in analytical chemistry…

 Analytical Chemistry

Method of Standard Addition

JoVE 10201

Source: Laboratory of Dr. Paul Bower - Purdue University


The method of standard additions is a quantitative analysis method, which is often used when the sample of interest has multiple components that result in matrix effects, where the additional components may either reduce or enhance the analyte absorbance signal. That results in…

 Analytical Chemistry

Overview of Biosensing

JoVE 5794

Biosensors are devices that use a wide range of biological processes and physical properties in order to detect either a biological molecule, such as a protein or cell, or a non-biological molecule, such as a chemical component or contaminant. This interdisciplinary field utilizes electrical, optical, electrochemical, or even mechanical properties to detect the presence…

 Bioengineering

Determining the Mass Percent Composition in an Aqueous Solution

JoVE 10172

Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry


Determining the composition of a solution is an important analytical and forensic technique. When solutions are made with water, they are referred to as being aqueous, or containing water. The primary component of a solution is referred to as…

 General Chemistry

Gas Chromatography (GC) with Flame-Ionization Detection

JoVE 10187

Source: Laboratory of Dr. B. Jill Venton - University of Virginia


Gas chromatography (GC) is used to separate and detect small molecular weight compounds in the gas phase. The sample is either a gas or a liquid that is vaporized in the injection port. Typically, the compounds analyzed are less than 1,000 Da, because it is difficult to…

 Analytical Chemistry

Removal of Trace Elements by Cupric Oxide Nanoparticles from Uranium In Situ Recovery Bleed Water and Its Effect on Cell Viability

1Division of Physical Therapy, Department of Orthopedics & Rehabilitation, University of New Mexico, 2Department of Ecosystem Science and Management, University of Wyoming, 3School of Pharmacy, University of Wyoming, 4Department of Environmental and Radiological Health Sciences, Colorado State University, 5Center for Environmental Medicine, Colorado State University, 6College of Pharmacy, California Northstate University

JoVE 52715

 Environment

Percutaneous Contrast Echocardiography-guided Intramyocardial Injection and Cell Delivery in a Large Preclinical Model

1Institute for Cardiovascular and Metabolic Research, School of Biological Sciences, University of Reading, 2Departamento de Medicina y Cirugía Animal, Facultad de Veterinaria, Universidad de Murcia, 3Institute of Virology and Immunology (IVI), 4Departamento de Anatomía y Anatomía Comparada, Facultad de Veterinaria, Universidad de Murcia, 5Unidad de Trasplante Hematopoyético y Terapia Celular, Departamento de Hematología, Hospital Universitario Virgen de la Arrixaca, IMIB, Universidad de Murcia

JoVE 56699

 Medicine

Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

1Department of Chemistry, Hunter College of the City University of New York, 2Ph.D. Program in Chemistry, Graduate Center of the City University of New York, 3Department of Radiology, Memorial Sloan Kettering Cancer Center, 4Department of Radiology, Weill Cornell Medical College

JoVE 59063

 Chemistry

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

1Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, 2Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute, University of Minnesota, 3Department of Botany and Zoology, Stellenbosch University

JoVE 57949

 Biochemistry

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

1Breast Surgery Group, Division of Medical Sciences and Graduate Entry Medicine, School of Medicine, Queen's Medical Centre, University of Nottingham, 2Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, 3Nottingham Digestive Diseases Centre and NIHR Nottingham Digestive Diseases Biomedical Research Centre, School of Medicine, Queen's Medical Centre, University of Nottingham

JoVE 57399

 Immunology and Infection

Empirical, Metagenomic, and Computational Techniques Illuminate the Mechanisms by which Fungicides Compromise Bee Health

1Vegetable Crop Research Unit, USDA-ARS, 2Department of Entomology, University of Wisconsin-Madison, 3Department of Horticulture, University of Wisconsin-Madison, 4Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, 5Department of Bacteriology, University of Wisconsin-Madison, 6Laboratory of Genetics, Genome Center of Wisconsin, 7DOE Great Lakes Bioenergy Research Center, Wisconsin Energy Institute, 8J.F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison

JoVE 54631

 Environment
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