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Pilot Projects: Small-scale tests of methods and procedures to be used on a larger scale if the pilot study demonstrates that these methods and procedures can work.

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

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JoVE 56474


 JoVE In-Press

Preparation and Photoacoustic Analysis of Cellular Vehicles Containing Gold Nanorods

1Institute of Applied Physics, Italian National Research Council, 2Department of Experimental and Clinical Biomedical Sciences, University of Florence, Firenze, 3Department of Physics and Astronomy, University of Florence, Sesto Fiorentino, 4Department of Pharmacy and Biotechnology, University of Bologna

JoVE 53328


 Bioengineering

Ultrasound-guided Botulinum Toxin-A Injections: A Method of Treating Sialorrhea

1Clinical and Biological Sciences Department, Neurology Unit, University of Torino, San Luigi Gonzaga Hospital, 2Oncology Department, Radiology Unit, University of Torino, San Luigi Gonzaga Hospital, 3Clinical and Biological Sciences Department, Dietologic and Nutrition Unit, University of Torino, San Luigi Gonzaga Hospital

JoVE 54606


 Medicine

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Quantitative Magnetic Resonance Imaging of Skeletal Muscle Disease

1Institute of Imaging Science, Vanderbilt University, 2Department of Radiology and Radiological Sciences, Vanderbilt University, 3Department of Biomedical Engineering, Vanderbilt University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Department of Physical Medicine and Rehabilitation, Vanderbilt University, 6Department of Physics and Astronomy, Vanderbilt University

JoVE 52352


 Medicine

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Conscious and Non-conscious Representations of Emotional Faces in Asperger's Syndrome

1Institute of Statistical Science, Academia Sinica, 2Max Planck Institute for Human Cognitive and Brain Sciences, 3Department of Psychology, Fo Guang University, 4Department of Electrical Engineering, Fu Jen Catholic University, 5State Research Institute of Physiology and Basic Medicine, 6Novosibirsk State University, 7Imaging Research Center, Taipei Medical University

JoVE 53962


 Behavior

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A Novel Method for Involving Women of Color at High Risk for Preterm Birth in Research Priority Setting

1School of Nursing, University of California, San Francisco, 2UCSF California Preterm Birth Initiative, University of California, San Francisco, 3School of Medicine, University of California, San Francisco, 4San Francisco Black Infant Health Program, 5Homeless Prenatal Program

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JoVE 56220


 JoVE In-Press

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Pilot Testing

JoVE 10031

Source: Laboratories of Gary Lewandowski, Dave Strohmetz, and Natalie Ciarocco—Monmouth University

In any experiment researchers have the challenge of creating experiences for participants that are consistent (i.e., reliable) and authentic (i.e., valid). Yet there are many ways to manipulate any one variable. For example, if you want participants to feel sad, you can have them think of their own sad memory, watch a sad video, or read a sad story. Researchers must find the best way to operationalize a psychological construct in order to produce the most effective manipulation possible. Often, before running the main study, researchers will pilot test (i.e., try out) their manipulations to check their effectiveness. This video demonstrates how to operationalize the same independent variable (acute stress) in three different ways. Specifically, this study seeks to identify the best sound (static, ticking clock, or crying baby) to play during a difficult task (solving complex math problems) to optimally manipulate stress. Psychological studies often use higher sample sizes than studies in other sciences. A large number of participants helps to ensure that the population under study is


 Experimental Psychology

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High-throughput Parallel Sequencing to Measure Fitness of Leptospira Interrogans Transposon Insertion Mutants During Golden Syrian Hamster Infection

1Veterans Affairs Greater Los Angeles Healthcare System, 2Departments of Medicine, David Geffen School of Medicine at University of California Los Angeles, 3Departments of Urology, David Geffen School of Medicine at University of California Los Angeles, 4Departments of Microbiology, Immunology, and Molecular Genetics, University of California Los Angeles

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JoVE 56442


 JoVE In-Press

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Leveraging CyVerse Resources for De Novo Comparative Transcriptomics of Underserved (Non-model) Organisms

1BIO5 Institute, University of Arizona, 2The School of Plant Sciences, University of Arizona, 3Genetics GIDP, University of Arizona, 4Biology Department, University of Massachusetts Amherst, 5School of Animal and Comparative Biomedical Sciences, University of Arizona, 6CyVerse, University of Arizona

JoVE 55009


 Genetics

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Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota

JoVE 50762


 Biology

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