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RNA Interference: A gene silencing phenomenon whereby specific dsRNAs (RNA, Double-stranded) trigger the degradation of homologous mRNA (RNA, Messenger). The specific dsRNAs are processed into Small interfering rna (siRNA) which serves as a guide for cleavage of the homologous mRNA in the Rna-induced silencing complex. Dna methylation may also be triggered during this process.

RNA Interference

JoVE 10804

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the expression of a gene by binding to its messenger RNA (mRNA) transcript, preventing the protein from being translated.

This process occurs naturally in cells, often through the activity of microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to selectively deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used to suppress genes that are overactive in diseases such as cancer. First, double-stranded RNA with a sequence complementary to the targeted gene is synthesized. Different types of double-stranded RNA can be used, including short interfering RNA (siRNA) and small hairpin RNA (shRNA). shRNA is one strand of RNA that is folded over—creating a double-stranded RNA with a hairpin loop on one side—and is a precursor of siRNA. The double-stranded RNA is then introduced into cells by methods such as injection or delivery by vectors, such as modified viruses. If shRNA is used, RNase enzymes in the cell, such as Dicer, cleave it down to the shorter siRNA, removing the hairpin loop. The siRNA then binds to an enzyme called Argonaute, which is part of a complex called RISC (RNA-induced silencing complex). Here, the two strands of the siRNA separate. One floats away w

 Core: Gene Expression

RNAi in C. elegans

JoVE 5105

RNA interference (RNAi) is a widely used technique in which double stranded RNA is exogenously introduced into an organism, causing knockdown of a target gene. In the nematode, C. elegans, RNAi is particularly easy and effective because it can be delivered simply by feeding the worms bacteria that express double stranded RNA (dsRNA) that is complementary to a gene of interest. First,…

 Biology I

Drosophila Development and Reproduction

JoVE 5093

One of the many reasons that make Drosophila an extremely valuable organism is that the molecular, cellular, and genetic foundations of development are highly conserved between flies and higher eukaryotes such as humans. Drosophila progress through several developmental stages in a process known as the life cycle and each stage provides a unique platform for developmental…

 Biology I

An Introduction to Developmental Genetics

JoVE 5325

Development is the complex process through which a single-celled embryo transforms into a multicellular organism. Developmental processes are guided by information encoded in an organism's DNA, and geneticists are trying to understand how this information leads to a fully formed organism.


This video reviews seminal research in the field of developmental biology, including the…

 Developmental Biology

Invertebrate Lifespan Quantification

JoVE 5338

Many animals naturally stop growing upon reaching adulthood, after which they undergo aging or "senescence" until dying. The amount of time between an organism\'s birth and death is called its lifespan, which can be influenced by various biological and environmental factors. By exposing organisms to different growth conditions, scientists can better understand the factors affecting lifespan.…

 Developmental Biology

C. elegans Maintenance

JoVE 5104

Ceanorhabditis elegans has been, and is still, used to great success as a model organism for studying a variety of developmental, genetic, molecular and even physical phenomena. In order to use C. elegans to its full potential, proper care and attention to the basic maintenance of this powerful organism is essential.


In this video you will learn the basic…

 Biology I

An Introduction to Caenorhabditis elegans

JoVE 5103

Caenorhabditis elegans is a microscopic, soil-dwelling roundworm that has been powerfully used as a model organism since the early 1970’s. It was initially proposed as a model for developmental biology because of its invariant body plan, ease of genetic manipulation and low cost of maintenance. Since then C. elegans has rapidly grown in popularity and is now utilized…

 Biology I

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

1Department of Pediatric Oncology/Hematology, VU University Medical Center, 2Department of Hematology, VU University Medical Center, 3Department of Medical Oncology, VU University Medical Center, 4Department of Clinical Genetics, VU University Medical Center, 5Division of General and Transplant Surgery, Azienda Ospedaliera Universitaria Pisana, Universita’ di Pisa, 6Amsterdam Immunology and Rheumatology Center, VU University Medical Center, 7Princess Máxima Center for Pediatric Oncology, 8Cancer Pharmacology Lab, AIRC Start-Up Unit, University of Pisa, 9Institute of Nanoscience and Nanotechnology, CNR-Nano

JoVE 54714

 Cancer Research

Injecting Gryllus bimaculatus Eggs

1Colby College, 2Division of Evolutionary Developmental Biology, National Institute for Basic Biology, 3Department of Biological Sciences, National University of Singapore, 4Department Biology and Department of Neuroscience, Bowdoin College, 5Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology, Harvard University

JoVE 59726

 Developmental Biology

Genome-wide RNAi Screening to Identify Host Factors That Modulate Oncolytic Virus Therapy

1Children's Hospital of Eastern Ontario (CHEO) Research Institute, 2Department of Biology, Microbiology and Immunology, University of Ottawa, 3Department of Pediatrics, University of Ottawa, 4Department of Microbiology, Immunology and Infectious Diseases, Cumming School of Medicine, University of Calgary

JoVE 56913

 Cancer Research

Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments

1Département de biologie moléculaire, biochimie médicale et pathologie, Faculté de médecine, Centre de recherche sur le cancer de l'Université Laval, 2Oncology, Centre de recherche du CHU de Québec, Université Laval, 3Laboratoire d'études moléculaires des valvulopathies (LEMV), Groupe de recherche en valvulopathies (GRV), Quebec Heart and Lung Institute/Research Center, 4Department of Surgery, Université Laval

JoVE 54557

 Biology

The C. elegans Excretory Canal as a Model for Intracellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis in a Single Cell: labeling by GFP-fusions, RNAi Interaction Screen and Imaging

1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children, Harvard Medical School, 2College of Life Sciences, Jilin University, 3Faculty of Health Sciences, University of Macau

JoVE 56101

 Developmental Biology

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

1Focal Area Infection Biology, Biozentrum, University of Basel, 2Centre d’Immunologie de Marseille-Luminy, Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical, Universidad de Navarra, 4BioDataAnalysis GmbH

JoVE 54263

 Immunology and Infection

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation

1Department of Microbiology, New York University School of Medicine, 2Molecular Neurobiology Program, Skirball Institute for Biomolecular Medicine, New York University School of Medicine, 3Department of Otolaryngology, New York University School of Medicine, 4Department of Cell Biology, New York University School of Medicine, 5Department of Physiology and Neuroscience, New York University School of Medicine, 6Department of Psychiatry, New York University School of Medicine, 7Center for Neural Science, New York University School of Medicine

JoVE 3823

 Immunology and Infection

The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital, Harvard Medical School, 2College of Life Sciences, Jilin University, 3Faculty of Health Sciences, University of Macau

JoVE 56100

 Developmental Biology

Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

1Institute of Medical Science, University of Toronto, 2Keenan Research Centre in the Li Ka Shing Knowledge Institute, St. Michael's Hospital, 3Department of Laboratory Medicine and Pathobiology, University of Toronto, 4Department of Medicine, University of Toronto

JoVE 58478

 Bioengineering

Generation, Amplification, and Titration of Recombinant Respiratory Syncytial Viruses

1UMR 1173 Institut national de la santé et de la recherche médicale (INSERM), Université de Versailles St. Quentin, 2UR892 Institut national de la recherche agronomique (INRA), Unité de virologie et immunologie moléculaires, 3Assistance Publique–Hôpitaux de Paris (AP-HP), Hôpital Ambroise Paré, Laboratoire de Microbiologie

JoVE 59218

 Immunology and Infection

Establishment of Viral Infection and Analysis of Host-Virus Interaction in Drosophila Melanogaster

1The Joint Center for Infection and Immunity, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, 2CAS Key Laboratory of Molecular Virology and Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 3CAS Key Laboratory of Infection and Immunity (CASKLII), Institute of Biophysics, Chinese Academy of Sciences, 4Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences

JoVE 58845

 Immunology and Infection

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

1Post-Graduate Program in Veterinary Sciences, Federal Rural University of Rio de Janeiro, 2Department of Animal Parasitology, Federal Rural University of Rio de Janeiro, 3Department of Epidemiology and Public Health, Federal Rural University of Rio de Janeiro

JoVE 59899

 Immunology and Infection

Low-Cost Gait Analysis for Behavioral Phenotyping of Mouse Models of Neuromuscular Disease

1Department of Neurology, Duke University School of Medicine, 2Duke Center for Neurodegeneration & Neurotherapeutics, Duke University School of Medicine, 3Biomedical Sciences Graduate Program, University of California San Diego, 4Department of Neurobiology, Duke University School of Medicine, 5Department of Cell Biology, Duke University School of Medicine

JoVE 59878

 Behavior
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