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Method for Measurement of Viral Fusion Kinetics at the Single Particle Level

Article DOI: 10.3791/1484-v • 14:59 min • September 7th, 2009
September 7th, 2009 •
1, 1,2, 1
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, 2Howard Hughes Medical Institute, Harvard Medical School

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We present an in vitro, two-color fluorescence assay to visualize the fusion of single virus particles with a fluid target bilayer. By labeling viral particles with fluorophores that differentially stain the viral membrane and its interior, we are able to monitor the kinetics of hemifusion and pore formation.

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Viral Fusion Kinetics Single Particle Level Membrane Fusion Fusion Assay Fluorescence Assay Fluorphore Staining Ganglioside-containing Lipid Bilayer CCD Camera PH-dependent Fusion Hemifusion Pore Formation
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