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JoVE Journal Chemistry

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Journal (Chemistry)

A 'Plug and Play' Method to Create Water-dispersible Nanoassemblies Containing an Amphiphilic Polymer, Organic Dyes and Upconverting Nanoparticles

Use of Stopped-Flow Fluorescence and Labeled Nucleotides to Analyze the ATP Turnover Cycle of Kinesins

Journal (Chemistry)

Use of Stopped-Flow Fluorescence and Labeled Nucleotides to Analyze the ATP Turnover Cycle of Kinesins

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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D ¹H NMR

Article DOI: 10.3791/50747-v • 14:44 min • December 16th, 2013

December 16th, 2013 •

Michal S. Shoshan¹, Edit Y. Tshuva¹, Deborah E. Shalev²

¹Department of Chemistry, The Hebrew University of Jerusalem, ²Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem

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svenska (Swedish)

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The NMR-solution structure of a metallochaperone model peptide with Cu (I) was determined, and a detailed protocol from sample preparation and 1D and 2D data collection to a three-dimensional structure is described.

Tags

Peptide-metal Complexes 1D NMR 2D NMR Cu (I) Binding Metallochaperone Transport Proteins Copper Oxidation Redox Reactions Cu (I) Complex NMR Spectroscopy Solution Structures Proteins Peptides Crystallization X-ray Crystallography NMR Technique Sample Preparation Data Collection Peak Assignment Integration Molecular Mechanics Calculations Structure Analysis

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