Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Zachary A. Crannell; Brittany Rohrman; Rebecca Richards-Kortum

doi:10.3791/52620

Journal

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생물학

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내부 양성 대조군과 양적 재조합 효소 중합 효소 증폭 분석 개발

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

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JoVE 신문 생물학

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

내부 양성 대조군과 양적 재조합 효소 중합 효소 증폭 분석 개발

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13,512 Views

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08:37 min

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March 30, 2015

DOI:

10.3791/52620-v

DOI:

10.3791/52620-v

•

08:37 min

March 30, 2015

•

14 Views

Zachary A. Crannell*¹, Brittany Rohrman*¹, Rebecca Richards-Kortum¹

¹Department of Bioengineering,Rice University

Summary

Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.