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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
JoVE Journal
Biology
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JoVE Journal Biology
Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

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08:37 min

March 30, 2015

DOI:

08:37 min
March 30, 2015

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Summary

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Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.

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