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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

DOI:
10.3791/52620-v

•

08:37 min

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March 30, 2015

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, ,
1Department of Bioengineering,Rice University

Chapters

  • 00:05Title
  • 01:30Assembling the Quantitative Recombinase Polymerase Amplification (qRPA) Reactions for a Standards Curve
  • 05:16Constructing a Standard Curve from Multiple Experiments with Custom Scripts
  • 06:55Results: Construction of an HIV-1 DNA Standard Curve by qRPA
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.

Tags

DNA QuantificationRecombinase Polymerase Amplification (RPA)Quantitative RPA (qRPA) AssayInternal Positive Control (IPC)Isothermal AmplificationHIV-1 DNAPoint-of-care Diagnostics

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