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Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

DOI:
10.3791/55444-v

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12:29 min

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April 19, 2017

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, , , , , ,
1Aberdeen Fungal Group, MRC Centre for Medical Mycology, Institute of Medical Sciences,University of Aberdeen, 2Department of Medicine,Memorial Sloan-Kettering Cancer Center, New York, US

Chapters

  • 00:05Title
  • 00:54Aspergillus fumigatus Culture and Labeling
  • 04:28Isolation of Human Monocytes
  • 07:30Isolation of Human Neutrophils
  • 08:30Live Cell Video Microscopy
  • 09:31Results: In Vitro Evaluation of Primary Immune Response to Aspergillus Conidia
  • 11:11Conclusion

Summary

자동 번역

자동 번역

Here, we describe a protocol to assess antifungal activity of primary human immune cells in real-time using fluorescent Aspergillus reporter conidia in conjunction with live-cell video microscopy and flow cytometry. Generated data provide insight into host cell-Aspergillus interactions such as fungicidal activity, phagocytosis, cell migration and inhibition of fungal growth.

Tags

Live Cell ImagingAntifungal ActivityHuman NeutrophilsHuman MonocytesAspergillus FumigatusFluorescent Reporter ConidiaInnate Immune ResponsePhagocytosisCell MigrationFungal Growth Inhibition

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