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Journal / Cancer Research / Phosphopeptide Bereicherung gepaart mit…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Phosphopeptide Bereicherung gepaart mit quantitativen markierungsfreie Massenspektrometrie zu untersuchen, die Phosphoproteome bei Prostatakrebs
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Phosphopeptide Bereicherung gepaart mit quantitativen markierungsfreie Massenspektrometrie zu untersuchen, die Phosphoproteome bei Prostatakrebs

Article DOI: 10.3791/57996-v • 12:23 min • August 2nd, 2018
August 2nd, 2018 •
*1,2, *3, 4, 5, 1,2,3,6,7
1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 6Pharmacology, Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey
* These authors contributed equally

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Dieses Protokoll beschreibt ein Verfahren zum Extrahieren und Phosphopeptides von Prostatakrebs-Zelllinien oder Gewebe für eine Analyse der Phosphoproteome per Massenspektrometrie-basierte Proteomics zu bereichern.

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Cancer Research Ausgabe 138 Prostatakrebs Massenspektrometrie Proteomik Phosphoproteomics Phosphorylierung Kinasen Zelle
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