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Journal / Cancer Research / Phosphopeptide संवर्धन लेबल के साथ युग्मित मुक्त…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Phosphopeptide संवर्धन लेबल के साथ युग्मित मुक्त मात्रात्मक जन स्पेक्ट्रोमेट्री प्रोस्टेट कैंसर में Phosphoproteome की जांच करने के लिए
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Phosphopeptide संवर्धन लेबल के साथ युग्मित मुक्त मात्रात्मक जन स्पेक्ट्रोमेट्री प्रोस्टेट कैंसर में Phosphoproteome की जांच करने के लिए

Article DOI: 10.3791/57996-v • 12:23 min • August 2nd, 2018
August 2nd, 2018 •
*1,2, *3, 4, 5, 1,2,3,6,7
1Graduate Program in Cellular and Molecular Pharmacology, School of Graduate Studies, Rutgers University, The State University of New Jersey, 2Graduate Program in Quantitative Biomedicine, School of Graduate Studies, Rutgers University, The State University of New Jersey, 3Department of Medicine, Division of Medical Oncology, Rutgers Robert Wood Johnson Medical School, 4Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, Jonsson Comprehensive Cancer Center, UCLA Metabolomics Center, and California NanoSystems Institute, David Geffen School of Medicine, University of California, Los Angeles, 5Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, 6Pharmacology, Rutgers Robert Wood Johnson Medical School, 7Cancer Metabolism and Growth Program, Rutgers Cancer Institute of New Jersey
* These authors contributed equally

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इस प्रोटोकॉल का वर्णन एक प्रक्रिया को निकालने के लिए और प्रोस्टेट कैंसर कोशिका लाइनों या ऊतकों से phosphopeptides को समृद्ध phosphoproteome के माध्यम से मास स्पेक्ट्रोमेट्री आधारित प्रोटियोमिक् के विश्लेषण के लिए ।

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कैंसर रिसर्च अंक १३८ प्रोस्टेट कैंसर मास स्पेक्ट्रोमेट्री प्रोटियोमिक् phosphoproteomics फास्फारिलीकरण kinases सेल सिग्नलिंग
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