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In Vitro-Bewertung der onkogenen Transformation in menschlichen Mammaepithelzellen

Article DOI: 10.3791/61716-v • 09:44 min • September 24th, 2020
September 24th, 2020 •
1,2, 1,3,4, 5,6,7, 1, 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

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Dieses Protokoll bietet experimentelle In-vitro-Werkzeuge zur Bewertung der Transformation menschlicher Brustzellen. Detaillierte Schritte zur Nachverfolgung der Zellproliferationsrate, der verankerungsunabhängigen Wachstumskapazität und der Verteilung von Zelllinien in 3D-Kulturen mit Kellermembranmatrix werden beschrieben.

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Krebsforschung Ausgabe 163 Zelltransformation Wachstumskurve Verankerungstest 3D-Zellkultur menschliche Brustepithelzellen Tumorigenese
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