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Journal / Bioengineering / Kultivierung einer dreidimensional…
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Bioengineering

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JoVE Journal Bioengineering
Kultivierung einer dreidimensional rekonstruierten menschlichen Epidermis im großen Maßstab
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Kultivierung einer dreidimensional rekonstruierten menschlichen Epidermis im großen Maßstab

Article DOI: 10.3791/61802-v • 08:49 min • May 28th, 2021
May 28th, 2021 •
1, 1,2, 3, 3,4, 1, 2
1Adolphe Merkle Institute, University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute, North Carolina State University

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Dieses Protokoll beschreibt eine einfache Methode, um dreidimensionale rekonstituierte menschliche Epidermis reproduzierbar und robust zu kultivieren. Zusätzlich charakterisiert es die Struktur-Funktions-Beziehung des epidermalen Barrieremodells. Die biologischen Reaktionen der rekonstituierten menschlichen Epidermis auf proinflammatorische Reize werden ebenfalls vorgestellt.

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Bioengineering Rekonstituierte humane Epidermis humane epidermale Äquivalente primäre Keratinozyten Epidermis In-vitro-Studien.
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