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Journal / Biochemistry / Экстракция молекул гликогена печени для…
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Biochemistry

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JoVE Journal Biochemistry
Экстракция молекул гликогена печени для определения структуры гликогена
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Экстракция молекул гликогена печени для определения структуры гликогена

Article DOI: 10.3791/63088-v • 04:50 min • February 8th, 2022
February 8th, 2022 •
1,2, 3,4, 3,5, 1,2,6, 7
1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute

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Определена оптимальная концентрация сахарозы для экстракции гликогена печени с помощью центрифугирования градиента плотности сахарозы. Добавление 10-минутной стадии кипячения для ингибирования ферментов, расщепляющих гликоген, оказалось полезным.

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В этом месяце в JoVE выпуск 180 Определение структуры гликогена полимер глюкозы уровень сахара в крови свойства гликогена метод экстракции градиентное центрифугирование плотности сахарозы молекулярное повреждение плотность раствора сахарозы размеры частиц гликогена стадия кипения денатурация ферментов разрушающих гликоген
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