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JoVE Journal Biology
Laser mikrodissektion til artsagnostiske enkeltvævsapplikationer
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Laser mikrodissektion til artsagnostiske enkeltvævsapplikationer

Article DOI: 10.3791/63666-v • 08:57 min • March 31st, 2022
March 31st, 2022 •
1,2, 1, 1, 3, 1
1Center for Developmental Genetics, New York University, 2Sacred Heart University, 3Baylor School

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En protokol er beskrevet, der bruger lasermikrodissektion til at isolere individuelle nematodevæv til RNA-sekventering. Protokollen kræver ikke artsspecifikke genetiske værktøjssæt, der gør det muligt at sammenligne genekspressionsprofiler mellem forskellige arter på niveau med enkeltvævsprøver.

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