29,629 Views
•
11:42 min
•
December 28, 2015
DOI:
Podsumowanie
Automatically generated
Laboratory-scale production of eukaryotic proteins with appropriate post-translational modification represents a significant barrier. Here is a robust protocol with rapid establishment and turnaround for protein expression using a mammalian expression system. This system supports selective amino acid, selective labeling of proteins and small molecule modulators of glycan composition.
Powiązane Filmy
09:40
Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
Powiązane Filmy
19463 Views
07:47
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Powiązane Filmy
52959 Views
12:55
Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
Powiązane Filmy
24039 Views
09:46
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Powiązane Filmy
45819 Views
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
Powiązane Filmy
32273 Views
14:20
Absolute Quantum Yield Measurement of Powder Samples
Powiązane Filmy
27883 Views
07:44
High-throughput Purification of Affinity-tagged Recombinant Proteins
Powiązane Filmy
14125 Views
07:50
Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
Powiązane Filmy
48291 Views
12:16
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
Powiązane Filmy
24017 Views
12:54
Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice
Powiązane Filmy
28339 Views
Read Article
Cytuj ten artykuł
Subedi, G. P., Johnson, R. W., Moniz, H. A., Moremen, K. W., Barb, A. W. High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension. J. Vis. Exp. (106), e53568, doi:10.3791/53568 (2015).
Copy