9,275 Views
•
10:00 min
•
February 17, 2017
DOI:
Transkrypcja
Automatically generated
Podsumowanie
Automatically generated
Studying ion channels through a heterologously expressing system has become a core technique in biomedical research. In this manuscript, we present a time efficient method to achieve tightly controlled ion channel expression by performing transient transfection under the control of an inducible promoter.
Powiązane Filmy
06:21
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Powiązane Filmy
6413 Views
08:35
Inducible and Reversible Dominant-negative (DN) Protein Inhibition
Powiązane Filmy
8312 Views
08:51
Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Powiązane Filmy
9138 Views
15:28
Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
Powiązane Filmy
17388 Views
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
Powiązane Filmy
32317 Views
13:07
One-channel Cell-attached Patch-clamp Recording
Powiązane Filmy
24211 Views
11:32
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
Powiązane Filmy
13197 Views
12:09
Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
Powiązane Filmy
10656 Views
07:28
An Introduction to Transfection
Powiązane Filmy
170291 Views
02:09
Indukowalny system regulowany Tet-Off: metoda in vitro do modulacji ekspresji genów w hodowanych komórkach przy użyciu systemu Tetracyklina-Off
Powiązane Filmy
1108 Views
Read Article
Cytuj ten artykuł
Geron, M., Hazan, A., Priel, A. Controllable Ion Channel Expression through Inducible Transient Transfection. J. Vis. Exp. (120), e55370, doi:10.3791/55370 (2017).
Copy