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March 28, 2016
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Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is presented.
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Kang, J., Kawaguchi, D., Wang, L. Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons. J. Vis. Exp. (109), e53818, doi:10.3791/53818 (2016).
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