LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

Andrew J. Worth; Dylan M. Marchione; Robert C. Parry; Qingqing Wang; Kevin P. Gillespie; Noelle N. Saillant; Carrie Sims; Clementina Mesaros; Nathaniel W. Snyder; Ian A. Blair

doi:10.3791/53941

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LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

JoVE Journal
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LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism

11,102 Views

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06:04 min

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April 04, 2016

DOI:

10.3791/53941-v

DOI:

10.3791/53941-v

•

06:04 min

April 04, 2016

•

7 Views

Andrew J. Worth*^1,2, Dylan M. Marchione*^2,3, Robert C. Parry^1,2, Qingqing Wang^2,3, Kevin P. Gillespie^2,3, Noelle N. Saillant⁴, Carrie Sims⁴, Clementina Mesaros^1,2, Nathaniel W. Snyder⁵, Ian A. Blair^1,2

¹Center for Cancer Pharmacology,University of Pennsylvania, ²Center for Excellence in Environmental Toxicology,University of Pennsylvania, ³Penn SRP and Department of Systems Pharmacology and Translational Therapeutics,University of Pennsylvania, ⁴Division of Traumatology, Department of Surgery, Critical Care and Acute Care Surgery,University of Pennsylvania, ⁵A.J. Drexel Autism Institute,Drexel University

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Podsumowanie

Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.