14,928 Views
•
11:30 min
•
January 26, 2017
DOI:
Podsumowanie
Automatically generated
We describe here a novel, robust, and efficient tandem affinity purification (TAP) method for the expression, isolation, and characterization of protein complexes from eukaryotic cells. This protocol could be utilized for the biochemical characterization of discrete complexes as well as the identification of novel interactors and post-translational modifications that regulate their function.
Powiązane Filmy
10:37
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
Powiązane Filmy
14915 Views
09:35
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Powiązane Filmy
13789 Views
08:23
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Powiązane Filmy
11321 Views
07:01
Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag
Powiązane Filmy
10204 Views
10:02
Identification of Protein Interacting Partners Using Tandem Affinity Purification
Powiązane Filmy
37494 Views
14:58
Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
Powiązane Filmy
48230 Views
13:51
The MultiBac Protein Complex Production Platform at the EMBL
Powiązane Filmy
15910 Views
10:36
Identifying Protein-protein Interaction in Drosophila Adult Heads by Tandem Affinity Purification (TAP)
Powiązane Filmy
13875 Views
10:36
Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method
Powiązane Filmy
9504 Views
02:05
Tandem Affinity Purification Assay to Study Protein-Protein Interactions
Powiązane Filmy
242 Views
Read Article
Cytuj ten artykuł
Ma, Z., Fung, V., D'Orso, I. Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells. J. Vis. Exp. (119), e55236, doi:10.3791/55236 (2017).
Copy