A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Sanat Upadhyay; Leoncio Vergara; Pranjali Shah; Jan-&#197;ke Gustafsson; Ioannis Kakadiaris; Maria Bondesson

doi:10.3791/60321

Dziennik

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Biologia Rozwoju

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A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

JoVE Journal
Biologia Rozwoju

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JoVE Journal Biologia Rozwoju

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

8,877 Views

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08:55 min

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January 14, 2020

DOI:

10.3791/60321-v

DOI:

10.3791/60321-v

•

08:55 min

January 14, 2020

•

13 Views

Sanat Upadhyay¹, Leoncio Vergara², Pranjali Shah², Jan-Åke Gustafsson^3,4, Ioannis Kakadiaris^1,4, Maria Bondesson⁵

¹Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics,University of Houston, ²Institute of Biosciences and Technology,Texas A&M Health Science Center, ³Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling,University of Houston, ⁴Department of Biosciences and Nutrition,Novum, Karolinska Institutet, ⁵Department of Intelligent Systems Engineering,Indiana University

Transkrypcja

Podsumowanie

This article describes a method to mount fragile zebrafish embryos for extended time-lapse confocal microscopy. This low-cost method is easy to perform using regular glass-bottom microscopy dishes for imaging on any inverted microscope. The mounting is performed in layers of agarose at different concentrations.