Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

Vanessa C. C. Luz; Z&#233;lia Silva; Patr&#237;cia Sobral; Ankit Tanwar; Rachel L. Paterson; Paula A. Videira

doi:10.3791/65525

Dziennik

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Immunologia i Infekcje

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Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

JoVE Journal
Immunologia i Infekcje

This content is Free Access.

JoVE Journal Immunologia i Infekcje

Generation of Monocyte-Derived Dendritic Cells with Differing Sialylated Phenotypes

3,348 Views

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13:36 min

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October 20, 2023

DOI:

10.3791/65525-v

DOI:

10.3791/65525-v

•

13:36 min

October 20, 2023

•

33 Views

Vanessa C. C. Luz^1,2, Zélia Silva^1,2, Patrícia Sobral^1,2,3, Ankit Tanwar^4,5, Rachel L. Paterson⁶, Paula A. Videira^1,2,7

¹Associate Laboratory i4HB – Institute for Health and Bioeconomy, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ²UCIBIO – Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa, ³LAQV and REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,Universidade NOVA de Lisboa, ⁴Department of Microbiology and Immunology,Albert Einstein College of Medicine, ⁵Department of Oncology,Albert Einstein College of Medicine, ⁶Stemmatters, Biotecnologia e Medicina Regenerativa SA, Parque de Ciência e Tecnologia Avepark,Zona Industrial da Gandra, ⁷CDG & Allies – Professionals and Patient Associations International Network (CDG & Allies – PPAIN), Department of Life Sciences, NOVA School of Science and Technology,Universidade NOVA de Lisboa

Transkrypcja

Podsumowanie

A unique, comprehensive protocol to generate de-sialylated human monocyte-derived dendritic cells (mo-DCs) from isolated peripheral blood mononuclear cells (PBMCs) using a sialidase treatment is presented. Further, methods to assess the phenotypic and functional characterization of mo-DCs and evaluate how sialidase treatment improves the maturation level of mo-DCs are described.