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Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples
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Collection, Isolation, and Flow Cytometric Analysis of Human Endocervical Samples

Article DOI: 10.3791/51906-v • 12:35 min • July 6th, 2014
July 6th, 2014 •
1, 1, 1, 1,2
1Department of Medical Microbiology, University of Manitoba, 2Department of Community Health Sciences, University of Manitoba

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The use of cytobrush sampling to collect lymphocytes and monocytes from the endocervix is a minimally invasive technique that provides samples for analysis of female genital tract immunity. In this protocol, we describe the collection of cytobrush samples and immune cell isolation for flow cytometry assays.

Tags

Collection Isolation Flow Cytometric Analysis Human Endocervical Samples Mucosal Pathogens Mucosal Immunity Female Genital Tract (FGT) Heterosexual Transmission Sexually Transmitted Infections (STIs) Interplay Between Host And Pathogen Genital Mucosa Collection Techniques Cervical Cytobrush Samples Cervical Mononuclear Cells (CMC) Ex Vivo Flow Cytometry-based Immunophenotyping CMC Lymphocyte/monocyte Population Frequencies And Phenotypes Cervical-vaginal Lavage (CVL) Soluble Immune Mediators Cytokines Chemokines Anti-proteases Anti- Or Pro-inflammatory Environment
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