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Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

Article DOI: 10.3791/55162-v • 10:14 min • January 11th, 2017
January 11th, 2017 •
1, 2, 1,3
1Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, 2Department of Environmental Health, Fay W. Boozman School of Public Health, University of Arkansas for Medical Sciences, 3Surgical Service, Central Arkansas Veterans Healthcare System

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The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.

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Inter-chromosomal Stable Aberrations Multiple Fluorescence In Situ Hybridization (mFISH) Spectral Karyotyping (SKY) Irradiated Mice Molecular Cytogenetic Method Bone Marrow Cells Total Body Irradiation Radiation Biology Chromosomal Aberrations Genetic Damages Cell Generations Single Cell Suspension Bone Marrow Mononuclear Cell Separation Medium Metaphase Cell Spreads
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