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Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Article DOI: 10.3791/55578-v • 11:08 min • May 5th, 2017
May 5th, 2017 •
1, 2,3,4,5, 2, 1
1Flow Cytometry Core Facility, Center for Translational Research-Technical Core, Institut Pasteur, 2Unit for Lymphopoiesis, Immunology Department, INSERM U1223, University Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Institut Pasteur, 3Stem-Cell Microenvironments in Repair/Regeneration Team, Instituto de Investigação e Inovação em Saúde (i3s), INEB - Instituto de Engenharia Biomédica, 4ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, 5Stem Cells and Regenerative Medicine Team, UMRS 1166, ICAN - Institute of Cardiometabolism And Nutrition, UPMC - Université Pierre et Marie Curie - Paris 6, INSERM

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This article describes spectral cytometry, a new approach in flow cytometry that uses the shapes of emission spectra to distinguish fluorochromes. An algorithm replaces compensations and can treat auto-fluorescence as an independent parameter. This new approach allows for the proper analysis of cells isolated from solid organs.

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Spectral Flow Cytometry Cell Suspensions Solid Tissues Fluorochromes Emissions Spectra Autofluorescence Analysis Rare Cell Populations Parameters Immunology Stem Cell Biology Organs Large Number Of Parameters Preparation Intestine Heart Lung Skeletal Muscle Liver Fat Kidney Peyer's Patches
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