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Economical and Efficient Protocol for Isolating and Culturing Bone Marrow-derived Dendritic Cells from Mice

Article DOI: 10.3791/63125-v • 04:29 min • July 1st, 2022
July 1st, 2022 •
*1, *1, 1, 2, 1, 3
1Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, 2Department of Pathology, The Second Hospital of Tianjin Medical University, 3Department of Urology, The Affiliated Wuxi No.2 People’s Hospital of Nanjing Medical University
* These authors contributed equally

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Here, we present an economical and efficient method to isolate and generate high-purity bone marrow-derived dendritic cells from mice after 7 days of culture with 10 ng/mL GM-CSF/IL-4.

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Economical Protocol Efficient Protocol Isolating Dendritic Cells Culturing Dendritic Cells Bone Marrow-derived Dendritic Cells Mice Animal Subjects Animal Ethics Committee Tumor Immunity Research DC Cells Bone Marrow Differentiation Isolation Of Bone Marrow Preparation Of BM Cells Mouse Operating Table Femoral Artery Muscles Femur Alcohol Rinse Injection Culture BMDC
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