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Basic Methods in Cellular and Molecular Biology
Protein Detection with ELISA
Description
ELISA is a protein detection test that uses antibodies and a color change to identify a target in a sample. ELISA stands for enzyme-linked immunosorbent assay. It is called an immunoassay because antibodies do the detecting.
The test is carried out in a microtiter plate with small wells. In many ...
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Transcript
The ELISA, or enzyme-linked immunosorbent assay, is a widely used method for determining the presence or absence of a specific target protein.
Via a series of washing and binding steps, an antibody conjugated, or linked, to an enzyme will recognize a target protein at the bottom of a 96-well plate. When substrate is ad...
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