This is a free preview. For full access
Basic Methods in Cellular and Molecular Biology
The ELISA Method
Description
An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible by antibodies, which make the ELISA an immunoassay. Through a series of incubation and washing steps, these antibodies, which are ...
Show More
Transcript
The ELISA, or enzyme-linked immunosorbent assay, is a widely used method for determining the presence or absence of a specific target protein.
Via a series of washing and binding steps, an antibody conjugated, or linked, to an enzyme will recognize a target protein at the bottom of a 96-well plate. When substrate is ad...
Show More
Tags
More videos by Standards
Show More