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Chapters
- 00:05Title
- 01:34Experiment Day 0: Sample Preparation and ELISA Test Plate Coating with Capture Antibody for IL-2Ralpha, REG3alpha and HGF
- 02:48Experiment Day 1: IL-2Ralpha and REG3alpha ELISAs
- 04:13Experiment Day 1: Elafin and TNFR1 Test Plate Coating with Capture Antibody
- 04:39Experiment Day 1-2: HGF ELISA
- 05:46Experiment Day 2: Elafin ELISA
- 06:16Experiment Day 2: TNFR1 ELISA
- 06:45Experiment Day 2: IL-8 ELISA
- 07:25Results: Representative Optical Densities for the Standard Curve of 7 Different ELISA Plates Measuring REG3alpha Concentrations
- 08:06Conclusion
High throughput validation of multiple candidate biomarkers can be performed by sequential ELISA in order to minimize freeze/thaw cycles and use of precious plasma samples. Here, we demonstrate how to sequentially perform ELISAs for six different validated plasma biomarkers1-3 of graft-versus-host disease (GVHD)4 on the same plasma sample.
Tags
High Throughput Sequential ELISAValidation Of BiomarkersAcute Graft-Versus-Host DiseaseUnbiased Discovery Proteomics StrategiesNovel BiomarkersDiagnostic TestingPrognostic TestingTherapeutic InterventionsCandidate ProteinsValidate Candidate BiomarkersPatient Plasma SamplesAcute Graft-versus-host DiseaseAllogeneic Hematopoietic Stem Cell TransplantationELISAsGVHD ProteinsIL-2Rα5TNFR16HGF7IL-88Elafin2REG3α3PAP1Sequential ELISA PerformanceFreeze-thaw CyclesThawed Plasma TimePlasma UsagePlasma Biomarker Concentrations
