Isolation of Genomic DNA from Mouse Tails

Protocol

1. Cut tail pieces (3mm) and mark ears.
2. Add 720 ml STE and 30 ml Proteinase K (10 mg/ml stock).
3. Incubate at 55°C on heating block and vortex every hour for 3 hours at top speed for 10 seconds.
4. Inactivate proteinase K at 70°C for 5 minutes.
5. Quench on ice for 5 minutes.
6. Centrifuge tail DNA for 10 minutes at full speed.
7. Decant into new tube containing 720 ml Isopropan....

Materials

List of materials used in this article

Name	Company	Catalog Number	Comments
STE	Buffer			100 mm Tris; pH 8.5 /5 mM EDTA /0.2% SDS /200 mM NaCl / in H2O
TE	Buffer			100 mM Tris; pH 5.0 /1 mM EDTA /10 mM NaCl