In addition to common laboratory equipment used for RNA and protein analyses and cell culture, the following materials and reagents are required for this protocol.
Name of the reagent
Biosafety Cabinet suitable for Biosafety Level 2 containment
PCR thermocycler
Ultracentrifuge
Anesthesia-induction chamber with isoflurane scavengers
Digital Vernier caliper
IVIS imaging system
Highly competent DH5a E. coli
EcoRI, BamHI, & HindIII restriction enzymes
T4 DNA Ligase
Third generation lentivirus packaging plasmids VSV-G, pRSV-Rev and pMDLg/pRRE
Stovall, D. B., Wan, M., Zhang, Q., Dubey, P., Sui, G. DNA Vector-based RNA Interference to Study Gene Function in Cancer. J. Vis. Exp. (64), e4129, doi:10.3791/4129 (2012).