Materials
| Name | Company | Catalog Number | Comments |
| 10 mL syringe | |||
| 5 mL syringe | |||
| 1 mL syringe with elongated tip | |||
| Petri dish | 60 mm | ||
| Syringe filters | Millex | 22 µm PVDF filter | |
| Capillaries (for omatidia separation) | Glass, 1.2 x 0.68 mm (~7.5 cm each) | ||
| Polyethylene Tubing | Becton Dickinson | 1.57 x 1.14 mm (35 cm) | |
| 2 small beakers | 50 mL or less | ||
| 2 paraffin film sheets | Parafilm M | ~5 x 5 cm | |
| Bath chamber | home-made | ||
| Cover slips | 22 x 22 mm No. 0 | ||
| Paraplast Plus | Sigma | Paraffin – polyisobutylene mixture | To glue the coverslip onto the bottom of the bath chamber |
| Ground | Warner Instruments | 64-1288 | Hybrid Assembly Ag-AgCl Wire Assembly |
| Headless micro dissection needle | Entomology, 12 mm | ||
| Micro dissecting needle holder | |||
| Vise | |||
| 2 fine tweezers + 1 rough tweezers | Dumont #5, Biology | 0.05 x 0.02 mm, length 110 mm, Inox | |
| Stereoscopic zoom Microscope | Nikon | SMZ-2B | |
| Cold light source | Schott | KL1500 LCD | |
| Filter (Color) for cold light source | Schott | RG620 | |
| Delicate wipers | Kimtech | Kimwipes | |
| Electrode holder | Warner Instruments | QSW-T10P | Q series Holders compatible with Axon amplifiers, straight body style |
| Silver Wire | Warner Instruments | 0.25 mm diameter, needs to be chloridized | |
| Micromanipulator | Sutter Instruments | MP 85 | Huxley-Wall Style Micromanipulator |
| Faraday cage | home made | Electromagnetic noise shielding and black front curtain | |
| Anti-vibration Table | Newport | VW-3036-OPT-01 | |
| Osilloscope | GW | GOS-622G | |
| Perfusion system | Warner Instruments | VC-8P | Pinch valve control system |
| Perfusion valve controller | Scientific instruments | BPS-8 | |
| Suction system | |||
| Amplifier | Molecular Device | Axopatch-1D | |
| Head-stage | Molecular Device | CV - 4 | Gain: x 1/100 |
| A/D converter | Molecular Device | Digidata 1440A | |
| Clampex | Molecular Device | 10 | software |
| pCLAMP | Molecular Device | 10 | software |
| Light source (Xenon Arc lamp) | Sutter Instruments | Lambda LS | |
| Light detector | home made | phototransistor | |
| Filter wheel and shutter controller | Sutter Instruments | Lambda 10-2 with a Uniblitz shutter | |
| Filters (Natural density filter) | Chroma | 6,5,4,3,2,1,0.5,0.3 | |
| Filter (Color) | Schott | OG590, Edge filter | |
| Xenon Flash Lamp system | Dr. Rapp OptoElecftronic | JML-C2 | |
| Light guide | Quartz | ||
| Pulse generator | AMPI | Master 8 | |
| Microscope | Olympus | IX71, Inverted | |
| Red illumination filter (Microscope) | RG630 / RG645 ø45mm | ||
| Microscope objective | Olympus | X60/0.9 UplanFL N air or X60/1.25 UplanFI oil | |
| CCD Camera | Andor | iXon DU885K | |
| NIS Element | Nikon | AR | software |
| Ca+2 indicator | Invitrogen | Calcium green 5N | |
| Excitation & emission filters and dichroic mirror | Chroma | 19002 - AT - GFP/FITC Longpass set | |
| Vertical pipette puller | Narishige | Model PP83 | Use either vertical or horizontal puller, as preferred. |
| Horizontal pipette puller | Sutter Instrument | Model P-1000 Flaming/Brown Micropipette Puller | |
| Filament | Sutter Instrument | 3 mm trough or square box | |
| Capillaries | Harvard Apparatus | borosilicate glass capillaries | 1 x 0.58 mm |
References
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