This paper reports that the addition of Y-27632 to TIVA medium can significantly increase the yield of melanocytes from adult skin tissues.
The isolation and culture of primary melanocytes from skin tissues is very important for biological research and has been widely used for clinical applications. Isolating primary melanocytes from skin tissues by the conventional method usually takes about 3 to 4 weeks to passage sufficiently. More importantly, the tissues used are usually newborn foreskins and it is still a challenge to efficiently isolate primary melanocytes from adult tissues. We recently developed a new isolation method for melanocytes that adds Y-27632, a Rho kinase inhibitor, to the initial culture medium for 48 h. Compared with the conventional protocol, this new method dramatically increases the yield of melanocytes and shortens the time required to isolate melanocytes from foreskin tissues. We now describe this new method in more detail using adult epidermis to efficiently culture primary melanocytes. Importantly, we show that melanocytes obtained from adult tissues prepared by this new method can function normally. This new protocol will significantly benefit studies of pigmentation defects and melanomas using primary melanocytes prepared from easily accessed adult skin tissues.
The goal of this study was to develop a simple and effective protocol to isolate melanocytes from the epidermis of adult skin for biological research and clinical applications. Melanocytes, which are located in the basal epidermis of the skin and in hair follicles, play an important role in pigmentation of the skin and hair by producing melanin1. The resulting skin pigmentation from epidermal melanocytes acts as an ultraviolet radiation filter that reduces/prevents DNA damage to underlying cells in the skin2. The abnormal proliferation of melanocytes in the skin is quite common, such as in the formation of benign nevi (moles) in which melanocytes potentially transform to oncogenic growth followed by cellular senescence3.
Since 1957, the isolation and subsequent culture of human primary melanocytes has been possible4, but only since 1982 has there been an efficient method that can reproducibly establish cultures of human melanocytes from the epidermis5. The conventional method to isolate primary melanocytes from the epidermis involves a two-step enzymatic digestion. Briefly, the skin is initially digested with dispase to separate the epidermis from the dermis, after which the epidermis is digested with trypsin to produce suspensions containing melanocytes and keratinocytes, which can then be selectively grown in different media. Currently, the initial culture usually takes about 3 to 4 weeks for melanocytes to reach confluency using the conventional method, which is likely due to the low efficiency of their isolation. Therefore, increasing the initial production of melanocytes from adult skin tissues would be quite helpful for both laboratory research and clinical applications.
Many growth factors, most of which have been shown to be secreted by keratinocytes (e.g., α-MSH, ACTH, bFGF, NGF, ET-1, GM-CSF, HGF, LIF and SCF)5–8, regulate the differentiation and proliferation of mammalian melanocytes. In the absence of feeder layers, the lack of those growth factors leads to the decreased proliferation of melanocytes and their increased apoptosis9. The co-culture of keratinocytes as feeder cells and melanocytes could lead to accelerated melanocyte proliferation and reduced apoptosis. However, the co-culture method not only demands more skin tissues to prepare keratinocytes, which is not practical, but also could not work efficiently because the culture conditions required by melanocytes does not favor keratinocyte growth, and vice-versa.
Previous studies have reported that the addition of Y-27632, a ROCK inhibitor, into the growth medium can enhance the yield of human primary epidermal cells from skin tissues10–14. Therefore, it would be interesting to test whether the isolation of human primary melanocytes would benefit from the presence of Y-27632. Indeed, the addition of Y-27632 into the inoculation TIVA medium15, which contains TPA, IBMX, Na3VO4 and dbcAMP, significantly increased the yield of primary melanocytes isolated from foreskin tissues in the initial cultures16. Compared to the conventional protocol, this new protocol is significantly more efficient in increasing the yield of melanocytes16. Therefore, this protocol describes the new method in detail using adult skin tissues based on a previous study16 in order to promote its application in basic and applied biological research.
The use of adult foreskin tissues in this protocol has been approved by the Human Research Ethics Committee (No.2015120401, date: May 12, 2015).
NOTE: Perform all the following procedures in a sterile environment to prevent contaminations of cells and cultures.
1. Preparations
2. The conventional method
3. The new method
NOTE: The procedure for tissue preparation and digestion in the new method is the same as described above for the conventional method, the only difference being that the isolated fresh cells are resuspended in 10 mL of TIVA medium containing 10 µM Y-27632.
4. Cell passaging
Figure 1 shows a schematic diagram comparing the conventional and the new methods. The procedure for tissue preparation and digestion with the new method is the same as the procedure for the conventional method, the only difference being that the isolated cells are resuspended in 10 mL of TIVA medium in the presence of 10 µM Y-27632. Two days after seeding, replace the media with normal TIVA medium without Y-27632. The conventional method of isolating primary melanocytes from skin tissues usually requires about 3 to 4 weeks for melanocytes to grow in sufficient numbers to passage, but more importantly, the tissues usually come from newborn babies and it is very difficult to isolate primary melanocytes from adult skin tissues. However, using the new method, melanocytes from adult tissues are observed in significantly greater numbers than melanocytes isolated using the conventional method. Microscopic views were taken at days 3, 6 and 8 when isolating the melanocytes from adult skin tissue using the conventional method and the new method (Figure 2A). In these microscopic views, the new method significantly increased the yield of melanocytes at days 6 and 8. Melanocyte numbers were then quantified by counting at least 10 different microscopic fields at days 3, 6 and 8. The results showed that the number of melanocytes isolated using the new method is much higher than the number of melanocytes isolated using the conventional method at days 6 and 8 (Figure 2B). After 8 days of culture, the melanocytes were harvested using trypsin and then counted with an automated cell counter in each single 100 mm dish, which revealed that the new method increased the melanocyte yield by about five times (Figure 2C). Microscopic views taken at day 9, one day after passaging, showed that the passaged melanocytes proliferated normally (Figure 2D), which was confirmed by Ki67 staining (Figure 2E).
The enhanced yield of melanocytes by Y-27632 during the initial culture after isolation has been shown through the regulation of keratinocytes in a previous publication16. As shown in Figure 3, Y-27632 did not increase the growth and proliferation of passaged melanocytes (Figure 3A-B) and did not inhibit the apoptosis of melanocytes (Figure 3C-D). However, Y-27632 significantly increased keratinocyte attachment (Figure 3E-H) and also promoted its surviving (Figure 3I) in the melanocyte-culture TIVA media. Either co-culture with keratinocytes in the presence of Y-27632 or the conditioned medium derived from Y-27632 treated keratinocytes could significantly enhance the growth of melanocytes (Figure 3J).
To further characterize the melanocytes isolated using the new method, MITF, a specific melanocyte marker, was analyzed using immunofluorescence (IF) staining to check the purity of melanocytes after initial passaging. Figure 4A shows that the percentage of cells expressing MITF at passage 1 was nearly 100%, indicating that pure melanocytes were obtained after the first passage by the new method, which is similar to the conventional method. To test whether melanocytes isolated using the new method can function normally, they were treated with forskolin (FSK) for 2 days in vitro, which induced the levels of pigmentation of melanocytes (Figure 4B). Taken together, these data suggest that the melanocytes isolated using the new method can function normally and can produce pigment.
Figure 1: Comparison of the new method and the conventional method. This scheme shows a comparison of the conventional method with the new isolation method. The only difference is that the isolated fresh cells are resuspended in 10 mL of TIVA medium in the presence of 10 µM Y-27632. Two days after seeding, the medium is replaced with normal TIVA medium without Y-27632. The new method usually achieves a density of melanocytes suitable for passaging at around day 8, while the conventional method usually takes about 20 days to grow sufficient numbers of melanocytes for passaging. Please click here to view a larger version of this figure.
Figure 2: The new isolation method increases the production of primary melanocytes. (A) Representative images of melanocytes prepared by the conventional method (top row) and by the new method (bottom row) at days 3, 6 and 8 after the initial inoculation. (B) Quantification of melanocyte numbers prepared using the conventional method and the new method by counting melanocyte numbers in at least 10 different microscopic fields (cell number/vision) at days 3, 6 and 8. All experiments were performed in triplicate and results are expressed as the average number of cells per microscopic field. (C) After culture for 8 days, melanocytes were harvested using trypsin and the number of melanocytes prepared using the conventional method and the new method were counted using a cell counting plate. The number of cells was calculated from triplicate experiments, and the results show the average number of cells per 100 mm dish. (D) After counting the number of melanocytes using an automated cell counter, melanocytes prepared using the conventional method or the new method were seeded in new dishes and microscopic photos were taken at day 9. (E) Melanocytes isolated using the new method at passage 0 or at passage 1 were fixed and stained with Ki67 antibody (red) and DAPI (nuclei, blue). (B-C), Student’s t-test was used to analyze differences between the new and the conventional method, ** P < 0.01, ***P < 0.005. All scale bars represent 100 µm. Please click here to view a larger version of this figure.
Figure 3: Y-27632 enhances melanocyte growth through regulation of keratinocytes. (A) Pure melanocytes (passage 3) were treated with 10 μM Y-27632 for 12, 24, 48, and 60 h and the number of melanocytes was analyzed by CCK-8 assay. (B) Pure melanocytes (passage 3) were treated with 10 μM Y-27632 for 48h and then were fixed for Ki67 staining. Quantification of percentage of Ki67 positive cells among total live cells indicated with DAPI nuclear staining. (C-D) Pure melanocytes (passage 3) were treated with 10 μM Y-27632 for 48 h and then were either stained for apoptotic cells with Annexin V-FITC and propidium iodide followed by FACS analysis (C) or fixed for TUNEL assay. (E) Isolated epidermis after trypsin digestion was plated with TIVA medium with or without Y-27632 (10 μM) and 2 days later immunofluorescence analysis of keratinocytes was performed with a pan-cytokeratin antibody (pan-ck). (F) Quantification of keratinocytes in the Y-27632-treated and control dishes from (E) as percentage of keratinocytes in a total of 500 cells counted. (G) Pure passage 3 keratinocytes were seeded in TIVA medium with or without Y-27632 (10 μM). Images of epidermal cells are shown at one day after seeding. (H) Quantification of attached keratinocytes in the Y-27632-treated and control dishes from (G) as the percentage of attached cells in a total of 5000 cells seeded. (I) Pure passage 3 keratinocytes were plated with TIVA medium in the presence or absence of Y-27632. The microscopic images were obtained at 24 h. (J) Left panel: Equal numbers of pure passage 3 melanocytes were plated with TIVA medium in 4 groups with addition(s) as indicated: 1) Y-27632 (10 µM) alone (Y in graph), 2) passage 3 keratinocytes alone , 3) keratinocytes and Y-27632 (10 µM) (K+Y), and 4) no addition (negative control, con). Relative fold changes in melanocyte proliferation compared to the negative control were determined by counting the number of melanocytes at 24 h and 48 h after plating. Right panel: Conditioned TIVA media were obtained from the 4 groups of passage 3 melanocyte cultures in (left panel) at 48 h after plating. Then, equal numbers of passage 3 melanocytes were plated in 4 groups, each with one of the conditioned media. Relative fold changes in melanocyte proliferation compared to the negative control were determined by counting the number of melanocytes at 24 h and 48 h after plating. (A-J) All experiments have been repeated for 3 times, *p<0.05, **p<0.01, ***p<0.005. All Bars in the images represent 100 µm. The figure has been modified from Mi et al.16. Please click here to view a larger version of this figure.
Figure 4: Characterization of melanocytes isolated using the new method. (A) Representative images of immunofluorescence staining of MITF (red) in melanocytes prepared using the new or the conventional method. DAPI stains nuclei (blue). (B) Cell pellets were collected from melanocytes prepared using the new method and were treated for 2 days with forskolin (FSK) or DMSO vehicle as a control (con). All scale bars represent 100 µm. Please click here to view a larger version of this figure.
The protocol described here was based on a recent publication16. Some attention should be paid to the following critical steps to achieve the best results with the new method. First, successful separation of the epidermis and the dermis is crucial. Cut the adult foreskin tissues into 3-4 mm wide strips using a scalpel blade to make the dispase work more thoroughly and easily to separate the epidermis from the dermis. Second, when separating those two layers, contamination of the dermis should be avoided since dermal fibroblasts can also grow in melanocyte culture medium. Third, the trypsin digestion step should be less than 30 min; otherwise, it will significantly decrease the viability of the isolated cells.
This new method significantly shortens the time needed for melanocyte isolation. Human epidermal melanocytes produce melanin, which protects the skin from solar irradiation damage. Eisinger and Marko proposed the first practical method for the culture of human melanocytes from the epidermis5. Most importantly, the tissues used usually come from newborn babies and it is very difficult to isolate primary melanocytes from adult skin tissues. The Rho-associated protein kinase inhibitor Y-27632 significantly improves the efficiency of epidermal stem cell isolation11–13. Additionally, we also reported a method for isolating human primary epidermal cells from skin tissues in the presence of Y-2763210,11. In this protocol, Y-27632 was shown to efficiently increase the yield of primary melanocytes. Using the conventional method has a low cell recovery rate and needs around 3 to 4 weeks of culture for melanocytes to grow in sufficient numbers to passage. This new method, in which Y-27632 is added to the TIVA growth medium, increased melanocyte yield by about five times, and the melanocytes obtained can proliferate normally. We also tested the new method using a commercial medium and found similar results. Regarding the mechanism involved, a recent study demonstrated that the effect of Y-27632 to enhance the efficiency of melanocyte isolation occurs mainly through keratinocytes16.
Importantly, we obtained pure melanocytes with normal function using the new method. The Passage 1 (P1) melanocytes were nearly all stained for MITF expression indicating that pure melanocytes are obtained after the first passage. Primary melanocytes with high growth potential are very important for biological research and clinical applications. The melanocytes isolated using the new method can be induced to pigment after treatment with forskolin, an activator of adenylate cyclase that increases cyclic AMP levels, indicating these melanocytes can function normally, which will be useful for studying pigmentation defects and melanoma14.
In summary, a highly efficient method was successfully established to isolate primary melanocytes from adult skin tissues. This improved method could contribute to provide sufficient numbers of melanocytes in a rapid manner for biological research and for clinical applications.
The authors have nothing to disclose.
This work was supported by The National Key Research and Development Program of China (2017YFA0104604), The General Program of National Natural Science Foundation of China (81772093), Military Logistics Research Project (AWS17J005), the Key Program of Shandong Province Natural Science Foundation (ZR2019ZD36) and The Key Research and Development Program of Shandong Province (2019GSF108107) to X.W. Guangdong Basic and Applied Basic Research Foundation (2019A1515110833) and The Fundamental Research Funds of Shandong University (2019GN043) to J.M.
Alexa fluor-594 donkey anti-mouse IgG | Thermo Fisher Scientific | A21203 | For Immunofluorescence |
Alexa fluor-594 donkey anti-rabbit IgG | Thermo Fisher Scientific | R37119 | For Immunofluorescence |
Cell Culture Dish | Eppendorf | 30702115 | For cell culture |
Cell Strainer | Corning incorporated | 431792 | Cell filtration |
15 mL Centrifuge Tube | KIRGEN | 171003 | For cell centrifuge |
50 mL Centrifuge Tube | KIRGEN | 171003 | For cell centrifuge |
CO2 Incubator | Thermo Scientific | 51026333 | For cell incubating |
Constant Temperature Shaker | Shanghai Boxun | 150036 | For water bath |
DAPI | Abcam | ab104139 | For Immunofluorescence |
Dispase | Gibco | 17105-041 | For melanocyte isolation |
DMEM | Thermo Scientific | C11995500 | Component of neutralization medium |
Fetal Bovine Serum | Biological Industries | 04-001-1AC5 | Component of neutralization medium |
Fluorescence microscope | Olympus | 5E44316 | For Immunofluorescence |
Forskolin | MCE | HY-15371 | Induce pigmentation |
Glutamine | Thermo Fisher Scientific | 25030081 | melanocyte culture medium |
Ham's F12 | Thermo Scientific | 11330032 | melanocyte culture medium |
Inverted microscope | Olympus | 5C42258 | For cell microscopic observation |
3-isobutyl-1-methyl xanthine (IBMX) | Sigma | 17018 | melanocyte culture medium |
mouse anti-human MITF | Abcam | ab12039 | For Immunofluorescence |
Na3VO4 | Sigma | S6508 | melanocytes culture medium |
N6,2'-O-dibutyryladeno-sine 3',5'-cyclic monophosphate (dbcAMP) | Sigma | D0627 | melanocyte culture medium |
12-O-tetradecanoyl phorbol-13-acetate (TPA) | Sigma | 79346 | melanocyte culture medium |
Penicillin Streptomycin | Thermo Scientific | 15140-122 | Antibiotics |
rabbit anti-human Ki-67 | Abcam | ab15580 | For Immunofluorescence |
Phosphate buffered solution | Solarbio Life Science | P1020-500 | Washing solution |
Sorvall ST 16R Centrifuge | Thermo Scientific | 75004380 | Cell centrifugation |
TC20TM automated cell counter | Bio-Rad | 1450102 | Automatic cell counting |
0.05% Trypsin | Life Technologies | 25300-062 | For melanocyte dissociation |
Y-27632 | Sigma | Y0503 | For melanocyte isolation |