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Bioengineering

调查气管中的应力松弛和失效反应

Published: October 18, 2022
doi:
10.3791/64245
, , , ,
1Department of Biomedical Engineering,Widener University, 2Drexel University College of Medicine, 3School of Biomedical Engineering, Science and Health Systems,Drexel University, 4Rosetree Media School District

Summary

本方案确定了猪气管的拉伸应力松弛和破坏特性。这些方法的结果可以帮助提高对气管粘弹性和衰竭阈值的理解，并有助于提高肺系统计算模型的能力。

Abstract

气管的生物力学特性直接影响气流，并有助于呼吸系统的生物功能。了解这些性质对于了解该组织中的损伤机制至关重要。该协议描述了一种实验方法，用于研究猪气管的应力松弛行为，该气管被预先拉伸至0%或10%应变300秒，然后进行机械拉伸载荷直至失效。本研究详细介绍了猪气管生物力学测试的实验设计、数据采集、分析和初步结果。使用该协议中提供的详细步骤和数据分析MATLAB代码，未来的研究可以研究气管组织的时间依赖性粘弹性行为，这对于了解其在生理，病理和创伤条件下的生物力学反应至关重要。此外，对气管生物力学行为的深入研究将极大地有助于改善相关医疗设备的设计，例如在手术过程中广泛使用的气管内植入物。

Introduction

尽管其在肺部疾病中起着关键作用，但最大的气道结构气管对其粘弹性的研究有限1.深入了解气管的时间依赖性粘弹性行为对于肺力学研究至关重要，因为了解气道特异性材料特性可以帮助推进肺病的损伤预防，诊断和临床干预的科学，肺病是美国第三大死亡原因2，3，4。

现有的组织表征研究已经报道了气管5，6，7，8的刚度特性。尽管时间依赖性机械反应在组织重塑中很重要，但对时间依赖性机械反应的研究很少，病理学9，10也改变了这种反应。此外，缺乏时间依赖性响应数据也限制了目前诉诸于使用通用本构定律的肺力学计算模型的预测能力。需要通过进行应力松弛研究来解决这一差距，这些应力松弛研究可以提供所需的材料特性，为气管的生物物理研究提供信息。目前的研究提供了测试方法，数据采集和数据分析的详细信息，以研究猪气管的压力松弛行为。

Protocol

所描述的所有方法都得到了德雷克塞尔大学机构动物护理和使用委员会（IACUC）的批准。所有尸体动物都是从位于美国宾夕法尼亚州的美国农业部（USDA）批准的农场获得的。本研究使用了一头雄性约克郡猪（3周龄）的尸体。 1. 组织采集 从批准的农场获取猪的尸体，并在安乐死后2小时内进行实验。将尸体放在冰上，直到组织收获完成，以确保新鲜的组织?…

Representative Results

图1显示了夹紧部位附近的失效组织和夹钳内组织的存在，确认在拉伸测试期间没有打滑。图2显示了在被测样品的拉伸测试期间观察到的各种失效部位，包括顶部或底部夹紧部位或沿组织长度。数据分析结果总结在图3-4和表1-2中。气管样品在轴向或圆周预拉伸至10%应?…

Discussion

很少有研究报告气管21，23的应力松弛特性。需要研究来进一步加强我们对气管组织的时间依赖性反应的理解。本研究报告提供了进行此类调查的详细步骤。然而，为了确保可靠的测试，必须确保方案内的以下关键步骤：（1）适当的组织水合作用，（2）圆周和纵向样品中的相似组织类型（软骨环和肌肉的数量）分布，（3）在没有预拉伸的情况下夹紧样?…

Disclosures

The authors have nothing to disclose.

Acknowledgements

本出版物中报道的研究得到了美国国立卫生研究院尤尼斯·肯尼迪·施莱佛国家儿童健康和人类发展研究所的支持，奖项编号为R15HD093024，美国国家科学基金会CAREER奖编号1752513。

Materials

Disposable safety scalpels Fine Science Tools Inc 10000-10
eXpert 7600 ADMET Inc. N/A Norwood, MA
Forceps  Fine Science Tools Inc 11006-12 and 11027-12 or 11506-12
Gauge Safe ADMET Inc. N/A Free Download
Image J NIH N/A Open Source
Proramming Software – MATLAB  Mathworks N/A version 2018A
Scissors  Fine Science Tools Inc 14094-11 or 14060-09
Sterile phosphate buffer solution  Millipore, Thomas Scientific MFCD00131855
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References

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Tags

TracheaStress-relaxationFailure ResponsePulmonary MechanicsLarynxTracheal TubeLongitudinal StripsFailure TestingLoad Relaxation DataMechanical FailureTensile Mechanical Loading

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Singh, A., Majmudar, T., Iyer, A., Iyer, D., Balasubramanian, S. Investigating Stress-relaxation and Failure Responses in the Trachea. J. Vis. Exp. (188), e64245, doi:10.3791/64245 (2022).
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